The Nucleocapsid (N) protein of SARS-CoV-2 is the key analyte in rapid antigen tests. Our team recently determined the first 3D structure of the N protein (48 kDa) using cryo-EM. Molecular modeling suggests high similarity in conserved regions of the N protein across all variants of concern — ensuring the effectiveness of lateral-flow tests. Overall, these structural tools can guide the design of new immune therapies as we plan for future variants and pandemics.