With cryo electron microscopy (CryoEM) in the spot light these days, it's important to be aware of it's different flavors, as well as the complimentary technologies that have developed in tandem. In this talk I will focus on the use of electron cryotomography to study molecules in their cellular context, the development of cryo-focused ion beam milling for imaging within mammalian cells and tissue, as well as cryo-fluorescence microscopy for targeting specific molecules within the cell. I will also touch on the current challenges to be overcome as cryogenic imaging moves into the future.
