Raman / PL / AFM

Raman spectroscopy is a light scattering analytical technique used to probe both organic and inorganic materials. The information from Raman spectroscopy can be used to identify chemistry, molecular structure, effects of bonding, stress/strain, and environment effects (temperature/humidity). A very wide variety of samples can be measured with this technique, including solids, liquids and gases. This is also a non-destructive technique. The scale at which information can be obtained also varies from 10s of nanometers to a few millimeters.

Technique Advantages

  • Non-destructive acquisition of chemical bonding information
  • Mapping of chemical information from 10s of nm to a few mm
  • Simultaneously acquire topographical information (AFM) and chemical information (Raman/PL)

Sample Requirements

  • Raman/PL measurements can be performed for Solids, Liquids and Gases
  • Typical sample size is a 100s of nanometers to 100s of micrometers, however we have sampling accessories that allow us to probe down to 10s of nanometers and up to ~1 meter.
  • Additional sample requirements vary widely depending upon the specific technique employed for analysis.

Instrumentation

Horiba LabRam – Lucy

Lasers:

  • 229nm 10mW (filter cutoff is 350cm-1)  
  • 364nm 20mW (filter cutoff is 200cm-1
  • 458nm 420mW (filter cutoff is 100cm-1
  • 488nm 150mW (filter cutoff is 50cm-1

Detector:              

  • UV optimized Back Illuminated detector (2048x512 pixel)

Objectives:

  • 10x NA 0.2 WD 10.6mm
  • 50x LWD NA 0.5 WD 10.6mm
  • 50x NA 0.75 WD 0.4mm
  • 74x UVI NA 0.66 WD 1.0mm
  • 100x NA 0.9 WD 0.2mm

Gratings:

  • 600 g/mm (spectral resolution ~ 5cm-1 @ 488nm)
  • 1800 g/mm (spectral resolution ~2.5cm-1 @ 488nm)
  • 2400 g/mm (spectral resolution ~0.9cm-1 @ 488nm)
  • 3600 g/mm (spectral resolution ~0.4cm-1 @ 488nm)

Horiba LabRam – Vlabinir

Lasers:                    

  • 532nm 110mW (notch filter + 10cm-1)  
  • 633nm 35mW (edge filter 50cm-1, notch filter + 10cm-1)
  • 785nm 120mW (filter cutoff is 50cm-1)                                      
  • 1064nm 1000mW (filter cutoff is 100cm-1)                             

Detector:              

  • Back Illuminated detector (2048x512 pixel)
  • LN2 cooled InGaAs array detector
  • Avalanche Photodiode Detector

Objectives:           

  • 10x NA 0.2 WD 10.6mm
  • 40x Water NA 0.8 WD 3.3mm
  • 50x NA 0.75 WD 0.4mm
  • 50x PL NA 0.8 WD 0.5mm
  • 100x NA 0.9 WD 0.2mm

Gratings:              

  • 150 g/mm (spectral resolution ~ 7cm-1 @ 532nm) 
  • 300 g/mm (spectral resolution ~4cm-1 @ 532nm)
  • 600 g/mm (spectral resolution ~2cm-1 @ 488nm)
  • 1800 g/mm (spectral resolution ~0.9cm-1 @ 488nm)

Coupled to AIST-NT AFM, for co-localized Raman-AFM and Tip Enhanced Raman Spectroscopy (TERS) measurements.

AIST-NT (Stage scanning AFM)

Scan Size: (X,Y,Z) (30um, 30um, 10um)

Objectives:

  • 100x NA 0.7 WD 6mm
  • 10x NA 0.3 WD 20mm

Scan Modes: Contact, Tapping, Spectroscopy Tapping (for TERS), Kelvin, Magnetic Force

Accessories

Both Raman instruments (Lucy and Vlabinir) are the same exact model. There are several accessories that can be installed on both systems. The following are these additional accessories:

-Polarized light measurement capabilities from 400-700nm

-Macro Adaption: Backscattered macro measurements at 180 and 90 degrees under the microscope

-Double Pass Macro: Cuvette holder for signal enhancement of liquid/gas measurements within visible range

-Sample Reference: In-line holder for arc lamp or cuvette of liquid to monitor and correct the slightest peak shifting throughout long experiments

-Motorized XY Stage Scanner: X = 135mm / Y = 90 mm with minimum step size of 50nm and repeatability of 1um

-DuoScan:

  1. Rastering mirror to average Raman spectrum from area larger than laser spot size
  2. Mapping capabilities for samples that do not fit on motorized XY stage.
  3. Large area mapping + Averaging to scan entire sample surface

-Particle Finder: Locate all particles in field of view and setup automated measurement, includes statistical analysis package for particles

-Multivariate Analysis Package: Perform variety of multivariate analysis of large spectral datasets

-Linkam Heating/Cooling Stage: Temperature range (-196oC to 600oC) with four point electrical probes