Infrared Spectroscopy (FTIR)

Micro-transmission Infrared map of lignin in a section of a plant root

Infrared spectrometry is useful for the identification of both organic and inorganic compounds. Aggregates of atoms (or functional groups) such as C=O, -NO2, C-N, and C-F; just to name a few, are all associated with characteristic infrared absorptions. Thus, infrared spectrometry is ideal for the identification of functional groups present within a sample. FT-IR capabilities within the MCL are geared towards the analysis of solids (organic, inorganic, and biological) in a variety of forms to include: fibers, thin films, microtome cuts, particles, powders, coatings, residues, monolayers, and monolithic solids. With the recent acquisition of an infrared microscope MCL now has FT-IR mapping/imaging capabilities along with the capacity to perform infrared microanalysis on samples down to ~10 microns in size.

Some examples of applications include:

  • In-situ studies of CO adsorption on catalyst surfaces using diffuse reflectance FT-IR spectroscopy  (DRIFTs)
  • Temperature  induced morphology changes in polymers using  in-situ attenuated total reflection (ATR)
  • Quantification of bio-molecules  in organic solvents using liquid transmission FT-IR
  • Mapping of bio-molecules I tissue sections using micro-FT-IR with a focal plane array detector
FT-IR Acessory Transmission
liquid  transmission cells Interchangeable spacers  and windows 
Temp controlled High presure cell Can  operate  at 0C- 200C  and up to 4.5 MPa  with ZnSe windows
Brewsters angle holder Allows rotation of sample 360 degrees
diamond compressin cell 1.5 mm diameter clear aperture
short path gas sampling cell 100 mm path length gas cell with KBr windows
Pellet press Hydraulic press for 7 mm KBr pellet preparation

ATR  
Harrick diamax Routine ATR analysis  with diamond ATR crystal, heated ATR  0C-175C
Harrick MVP pro Routine ATR analysis  with diamond and Znse crystals
Pike HATR Multibounce high sensitivity liquid ATR analysis
Harrick Horizon Multibounce high sensitivity liquid ATR analysis
Pike Veemax Variable angle ATR allowng depth profiling with Znse and Ge crystals with polarizer
Harrick  seagull Variable angle ATR allowng depth profiling with Znse and Ge crystals with polarizer
Harrick VariGATR Grazing angle ATR  with Ge ATR crystal with polarizer

Specular reflectance  
Pike Veemax Variable angle  specular reflectance with precise wire grid polarizer
Harrick  seagull Variable angle  specular reflectance  with polarizer
Diffuse reflectance  
Harrick seagull Variable angle specular and difuse reflectance acessory
Harrick praying mantis Diffuse reflectance with inert transfer vessel
High temp reaction cell In-situ diffuse reflectance from RT-700C
low temp reaction cell Can operate at 10-6 torr to 1 ktorr and at temperatures from -150°C to 600°C 

Micro-FT-IR  
transmission 15X, 20X and 30X  reflective objectives with polarizer
ATR 100 and 250 micron 20X  Ge objectives with polarizer
Reflectance 15X, 20X and 30X  reflective objectives, 15X grazing angle objective with polarizer