Fourier Transform Infrared Spectroscopy (FTIR)

Infrared spectrometry is useful for the identification of both organic and inorganic compounds. Aggregates of atoms (or functional groups) such as C=O, -NO2, C-N, and C-F; just to name a few, are all associated with characteristic infrared absorptions. Thus, infrared spectrometry is ideal for the identification of functional groups present within a sample. FT-IR capabilities within the MCL are geared towards the analysis of solids (organic, inorganic, and biological) in a variety of forms to include: fibers, thin films, microtome cuts, particles, powders, coatings, residues, monolayers, and monolithic solids. With the recent acquisition of an infrared microscope MCL now has FT-IR mapping/imaging capabilities along with the capacity to perform infrared microanalysis on samples down to ~10 microns in size.

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Sample Requirements
  • The detection limit for routine analysis is ~0.1 wt%; under ideal conditions greater sensitivity can be achieved.

  • Typically, only a few milligrams of material are needed for analysis.

  • Samples may be in liquid, solid, or gaseous form. MCL has a significant collection of sampling accessories. See below.

  • Additional sample requirements vary widely depending upon the specific technique employed for analysis.

FTIR Instrumentation
Bruker Vertex V80v, V80 and V70

The MCL has three high performance research grade FT-IR spectrometers. The V80 and V70 are equipped with a wide range of sampling accessories. Through the use of interchangeable optical components MCL has the ability to acquire data over the near-IR (12,000-4,000 cm-1), mid-IR (4,000-400 cm-1), and far-IR (680-30 cm-1) regions. Additionally, the V80 is a step-scan capable instrument for high temporal resolution spectrometry. The evacuable optics bench of the V80v vacuum FTIR spectrometer allow acquisition of data in the mid-IR and far-IR regions without the interference of atmospheric water and carbon dioxide.

Bruker Hyperion 3000 Microscope

The Hyperion 3000 FT-IR microscope enables the acquisition of mid-IR and near-IR spectra from very small (>10 µm) samples with diffraction limited spatial resolution. Various collection modes are available and include: transmission, specular reflectance, attenuated total reflectance, and reflection-absorption. The microscope is equipped with brightfield, darkfield, and fluorescence illumination as well as polarization capabilities to enhance contrast and facilitate sample visualization. In addition to single point measurements, the microscope possesses extensive mapping and imaging capabilities. Use of the single element MCT detector in conjunction with the computer controlled stage permits sample mapping with a positioning accuracy of ±3 µm . The 64 x 64 element focal plane array detector enables diffraction limited mid-IR imaging of areas as large as 340 x 340 µm.

Sampling Accessories
Transmission
  1. FT-IR microscopy, 15x and 20x objectives (w/polarizer)
  2. Brewster’s Angle Holder (w/polarizer)
  3. Harrick Temperature controlled liquid demountable cell
  4. Liquid Cell (many spacers/windows)
  5. Diamond Compression Cell
  6. Pellet Press (7mm die set)
  7. Gas Sampling Cell
Specular Reflectance
  1. FT-IR microscopy, 15x and 20x objectives (w/polarizer)
  2. FT-IR microscopy, 15x grazing angle objective (w/polarizer)
  3. Pike VeeMax II (variable angle/polarization)
  4. Harrick Seagull, variable angle with polarizer
Attenuated Total Reflectance
  1. FT-IR microscopy, 20x objective – Ge crystal
  2. Pike HATR, horizontal multiple reflection (liquid trough) 
  3. Harrick Horizon, Horizontal multiple reflection (ZnSe liquid trough)
  4. Harrick MVP Pro, single reflection (diamond and ZnSe crystals) [pdf]
  5. Harrick Diamax, with diamond crystal single reflection temperature control
  6. Pike VeeMax II variable angle with polarization
  7. Harrick Seagull, variable angle with polarization
  8. Harrick VariGATR, grazing angle with polarizer
Diffuse Reflectance
  1. Harrick Praying Mantis
  2. Ambient, air-tight, and high temperature reaction cell for use with Praying Mantis
  3. Low temperature reaction chamber for use with Praying Mantis