Confocal Raman
Instrumentation
WITec CRM200 (132 MRI Bldg)
Description
Resolution of the WITec Confocal Raman instrument depends upon the wavelength of excitation laser and the Numerical Aperture (NA) of the objective. Currently, there are three wavelengths available for use with the instrument: (1) 488-nm; (2) 514-nm; and (3) 633-nm. MCL offers a number of top quality objectives to choose from, depending upon the requirements of the application. The three most commonly used objectives are the 40X (NA = 0.6), 100X (NA= 0.9), 100X immersion-oil (NA = 1.25) providing lateral resolutions (using 488-nm excitation) of, 488-nm, 325-nm, and 235-nm, respectively.
In solids, Raman spectroscopy is used to examine the inelastic scattering of the incident laser light by the vibration of the constituent molecules. Photons, of several different wavelengths, are emitted as a result of this inelastic scattering and provide insight into the phonon modes of the sample. Raman spectroscopy is a non-destructive technique and the spectra at a single point can be collected in a few seconds. In addition, he WITec confocal Raman microscope can be used to capture two (2D) and three dimensional (3D) spectral images.
Sample Requirements/Specifications
- Sample requirements vary according to the technique chosen for analysis. Contact the MCL staff analyst for additional information or to discuss your samples.
Rates
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